ComplementTech B101說明書

簡單描達: GVBo is a basic buffer that can be used to make other traditional buffers used for complemenassays. Ca++ and Mg++ may be added to GVBo to make GVB++ for classical pathway and lectin pathway assays. MgEGTA may be added to GVBo for alternative pathway assays. EDTA may be added to G/Bo to prepare GVBE to inhibit complement activation. GVBo is also used for dilution of serum and othelassay components in many complement assays especially in alternative pathway assays (Morgan, B.P.(2000: Dodds,A.W.and Sim, R.B.(1997)).

應用:GVBo should be used to prepare Er and to wash them before their use in assays. GVBo is used to dilute all of the components of alternative pathway assavs. The Genera Description section above describes the use of GVBo to prepare GVB++ , MgEGTA for alternative pathway assays and EDTA to prepale GVBE. GVBo is also used for dilution of serum and other assay components in many complement assays especially in alternative pathway assays.

規格:

1 Liter

存儲溫度: +40C Avoid freezing which causes gelatin to gel. lf frozen, heat to redissolve gelatin.

存儲溶液: Veronal is used as the buffer because in the mid-1900s this was the only bulffer for pH range72-7 4 that did not chelate metal ions and did not to inhibit complement reactions as did other buffers. Sodium chloride is present to provide an isotonic environment so that cells do not lyse due to osmotic pressure. Gelatin is present to prevent loss of protein components due to adsorption onto tips or tubes duringdilutions and in the assays themselves. Azide is present to prevent bacterial growth.

參考文獻: Morgan, B.P.ed.(2000) Complement Methods and Protocols. Humana Press. Dodds,A.W. and Sim, R.B. editors (1997). Complement A Practical Approach (ISBN 019963539) Oxford University Press, Oxford.

商品關鍵詞: GVBo(GVB without Ca++ and Mg++),B101,CompTech

Concentration: 0.1 % gelatin, 5 mM Veronal, 145 mM NaCl, 0.025 % NaN3 ,pH 7.3

Form: Liquid

Preservative: Sterile filtered with 0.025% sodium azide as a bactericidal agent

Physical Characteristics: The concentration of gelatin in this buffer is below the concentration that formssolid gels. However, because of the gelatin is close to its gelling concentration at 40C some strings of gelatin form during standing at this temperature. They can be redissolved easily by heating to 37oC or by brief heating in a microwave oven.